DESCRIPTION MW= 59.8 kDa. Full length nsp14 (3′-to-5′ exonuclease) from SARS-CoV-2, expressed in E. coli. The protein consists in residues 1-527 (NCBI reference sequence YP_009725309.1).
SEQUENCE Numeration of the nsp14 from NCBI YP_009725309.1
10 20 30 40 50 60 AENVTGLFKD CSKVITGLHP TQAPTHLSVD TKFKTEGLCV DIPGIPKDMT YRRLISMMGF 70 80 90 100 110 120 KMNYQVNGYP NMFITREEAI RHVRAWIGFD VEGCHATREA VGTNLPLQLG FSTGVNLVAV 130 140 150 160 170 180 PTGYVDTPNN TDFSRVSAKP PPGDQFKHLI PLMYKGLPWN VVRIKIVQML SDTLKNLSDR 190 200 210 220 230 240 VVFVLWAHGF ELTSMKYFVK IGPERTCCLC DRRATCFSTA SDTYACWHHS IGFDYVYNPF 250 260 270 280 290 300 MIDVQQWGFT GNLQSNHDLY CQVHGNAHVA SCDAIMTRCL AVHECFVKRV DWTIEYPIIG 310 320 330 340 350 360 DELKINAACR KVQHMVVKAA LLADKFPVLH DIGNPKAIKC VPQADVEWKF YDAQPCSDKA 370 380 390 400 410 420 YKIEELFYSY ATHSDKFTDG VCLFWNCNVD RYPANSIVCR FDTRVLSNLN LPGCDGGSLY 430 440 450 460 470 480 VNKHAFHTPA FDKSAFVNLK QLPFFYYSDS PCESHGKQVV SDIDYVPLKS ATCITRCNLG 490 500 510 520 GAVCRHHANE YRLYLDAYNM MISAGFSLWV YKQFDTYNLW NTFTRLQ
ISOTOPIC ENRICHMENT none
SUPPLIED AS Protein solution. The concentration is calculated from the absorbance at 280nm (ε280 = 93625 M-1cm-1).
STORAGE -20°C. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
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